AN IMMOBILIZED ANTIBODY-BASED AFFINITY GRID STRATEGY FOR ON-GRID PURIFICATION OF TARGET PROTEINS ENABLES HIGH-RESOLUTION CRYO-EM

An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM

Abstract In cryo-electron Scales microscopy (cryo-EM), sample preparation poses a critical bottleneck, particularly for rare or fragile macromolecular assemblies and those suffering from denaturation and particle orientation distribution issues related to air-water interface.In this study, we develop and characterize an immobilized antibody-based a

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The Circulation and Politics of Knowledge: Climate Change and Livelihood Struggles in a Coastal Fishing Community

Exploring the role of knowledge circulation in everyday life could provide interesting insights on how different forms of knowledge shapes the lifeworld and subjective realities of people.In the context of everyday livelihood struggles of a coastal fishing community, this paper examines the nature of knowledge circulation at the local level and its

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Dengue viruses – an overview

Dengue viruses (DENVs) cause the most common arthropod-borne viral disease in man with 50–100 million infections per year.Because of the lack of a vaccine Serveware and antiviral drugs, the sole measure of control is limiting the Aedes mosquito vectors.DENV infection can be asymptomatic or a self-limited, acute febrile disease ranging in sev

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A computational study on mixed convection in a porous media filled and partially heated lid-driven cavity with an open side

Applications of mixed convection due to lid-driven cavities can be found in different engineering technologies.A computational study was conducted to explore the effects of different LADIES ACCESSORIES JEWELLERY parameters on mixed convection in a porous media filled lid-driven cavity with one side opening in the presence of heat generation.The cav

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